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Healing & Recovery
Preclinical

NAD+

NAD+

Also known as: NAD, Coenzyme NAD, Nicotinamide Adenine Dinucleotide

Overview

Key Facts

Primary Goal: Research and therapeutic applications of NAD+

Serves as essential substrate for sirtuin deacetylases (SIRT1-7) regulating gene expression. Fuels PARP enzymes for DNA single-strand break repair.

Dosing Information

Half-Life

~30-45 minutes (IV); tissue levels persist longer

Typical Dose

50–200 mg

Frequency

1-3x per week (subcutaneous) or 250-500 mg IV

Cycle Length

Continuous supplementation or 4-12 week cycles

Administration Routes:
subcutaneousintramuscularoralintravenous

Benefits

  • Activates sirtuins (SIRT1-7) for DNA repair and longevity pathways
  • Restores mitochondrial function and ATP production
  • Enhances cellular energy metabolism
  • Supports cognitive function and neuroprotection
  • Promotes DNA damage repair via PARP enzymes
  • Improves metabolic health and insulin sensitivity
  • May support healthy circadian rhythm regulation

Side Effects

Flushing, warmth, and tingling during IV infusionmild
Nausea or GI discomfort (especially at higher doses)mild
Chest tightness or shortness of breath during rapid IV pushmild
Injection site discomfort (subcutaneous)mild
Headache (transient)mild

Mechanism of Action

1

Serves as essential substrate for sirtuin deacetylases (SIRT1-7) regulating gene expression

2

Fuels PARP enzymes for DNA single-strand break repair

3

Acts as electron carrier in mitochondrial oxidative phosphorylation (Complex I)

4

Regulates CD38/NADase activity in immune and inflammatory signaling

5

Supports circadian clock gene expression via SIRT1-CLOCK interactions

Contraindications

Do not use this peptide if any of the following apply:

  • Active cancer (NAD+ may fuel rapidly dividing cells — consult oncologist)
  • Gout or hyperuricemia (NAD+ metabolism can elevate uric acid)
  • Pregnancy or breastfeeding
  • Caution with concurrent chemotherapy or immunotherapy

Storage & Reconstitution

Unreconstituted (Powder)

Temperature2–8°C (36–46°F)
DurationUp to 3 months

Reconstituted (Mixed)

Temperature2–8°C (36–46°F)
Duration2-4 weeks

Research Summary

Preclinical

NAD+ biology has exploded in research interest since the work of David Sinclair (Harvard) and Shin-ichiro Imai (Washington University) on sirtuins and ageing. Human studies have demonstrated that NAD+ precursors (NMN and NR) safely elevate blood NAD+ levels. Clinical trials show improvements in insulin sensitivity, muscle function in elderly subjects, and cardiovascular biomarkers. IV NAD+ is widely used in integrative medicine clinics for energy, cognitive support, and addiction recovery, though large-scale RCTs for the injectable form are still limited. The compound is central to the 'Information Theory of Ageing' proposed by Sinclair's lab.

Frequently Asked Questions

Common questions about NAD+

UK-Specific Information

Exclusive data points and guidance for UK residents using NAD+

UK Lab Testing

UK Lab Testing

Recommended labs: Medichecks, Thriva (£89-£149 for peptide safety panel)

Why this matters: UK-specific lab testing guidance not available on US competitor sites

Commonly Stacked With

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