What is the half-life of Acetyl Hexapeptide-3?

Acetyl Hexapeptide-3 has a half-life of ~3-5 hours (topical activity window), which is why it's typically administered Twice daily (topical application) for optimal therapeutic effect.

How do I reconstitute Acetyl Hexapeptide-3?

Add bacteriostatic water to the peptide vial slowly down the side. Typical reconstitution uses 2-3ml of water for a 5mg vial. Gently swirl, do not shake.

Anti-Ageing

Preclinical

Acetyl Hexapeptide-3

Also known as: Argireline, Acetyl Hexapeptide-8, SNAP-6

Overview

Key Facts

Primary Goal: A synthetic hexapeptide that mimics the N-terminal end of SNAP-25, a protein essential for SNARE complex formation and neurotransmitter release

A synthetic hexapeptide that mimics the N-terminal end of SNAP-25, a protein essential for SNARE complex formation and neurotransmitter release. By inhibiting this complex in a mechanism analogous to botulinum toxin, Argireline reduces the intensity of facial muscle contractions that cause expression lines — earning it the nickname 'topical Botox.' Half-Life ~3-5 hours (topical activity window) Typical Dose 5–10 mg Frequency Twice daily (topical application) Cycle Length Continuous daily use; initial effects within 2-4 weeks, full results at 8-12 weeks

Dosing Information

Half-Life

~3-5 hours (topical activity window)

Typical Dose

5–10 mg

Frequency

Twice daily (topical application)

Cycle Length

Continuous daily use; initial effects within 2-4 weeks, full results at 8-12 weeks

Administration Routes:

topical

Storage & Reconstitution

Unreconstituted (Powder)

Temperature15–25°C (59–77°F)

DurationUp to 3 months

Reconstituted (Mixed)

Temperature15–25°C (59–77°F)

Duration2-4 weeks

Research Summary

Preclinical

Developed by Lipotec (now part of Lubrizol), Argireline was introduced in 2002 and quickly became a bestselling cosmetic peptide. In vitro studies showed 41% inhibition of SNARE complex formation at 0.05 mM. A 30-day clinical study demonstrated a 30% reduction in wrinkle depth around the eyes. While less potent than injectable botulinum toxin, its non-invasive nature and good safety profile have made it one of the most commercially successful cosmetic peptides globally.