What is the half-life of Glutathione?

Glutathione has a half-life of ~10-15 minutes (IV); intracellular GSH persists for hours, which is why it's typically administered 1-3x per week (injectable) or daily (oral/liposomal) for optimal therapeutic effect.

How do I reconstitute Glutathione?

Add bacteriostatic water to the peptide vial slowly down the side. Typical reconstitution uses 2-3ml of water for a 5mg vial. Gently swirl, do not shake.

Anti-Ageing

Preclinical

Glutathione

Also known as: GSH, Reduced Glutathione, L-Glutathione, Master Antioxidant

Overview

Key Facts

Primary Goal: A tripeptide composed of glutamate, cysteine, and glycine — widely recognized as the body's master antioxidant

A tripeptide composed of glutamate, cysteine, and glycine — widely recognized as the body's master antioxidant. Glutathione is present in every cell and plays a central role in detoxification, immune defense, and protection against oxidative stress. Levels decline with age, chronic illness, and toxin exposure, making supplementation (especially via injectable routes that bypass poor oral bioavailability) highly popular in anti-aging and wellness medicine. Half-Life ~10-15 minutes (IV); intracellular GSH persists for hours Typical Dose 200–600 mg Frequency 1-3x per week (injectable) or daily (oral/liposomal) Cycle Length Continuous supplementation or 8-12 week cycles

Dosing Information

Half-Life

~10-15 minutes (IV); intracellular GSH persists for hours

Typical Dose

200–600 mg

Frequency

1-3x per week (injectable) or daily (oral/liposomal)

Cycle Length

Continuous supplementation or 8-12 week cycles

Administration Routes:

subcutaneousintramuscularoralintravenous

Storage & Reconstitution

Unreconstituted (Powder)

Temperature2–8°C (36–46°F)

DurationUp to 3 months

Reconstituted (Mixed)

Temperature2–8°C (36–46°F)

Duration2-4 weeks

Research Summary

Preclinical

Glutathione is one of the most researched molecules in biology, with over 170,000 PubMed-indexed publications. Clinical studies have demonstrated its role in Parkinson's disease (IV glutathione improved motor scores in pilot trials), non-alcoholic fatty liver disease (NAFLD), cystic fibrosis, and HIV/AIDS-related immune decline. Dermatological research confirms its skin-lightening effects, with a randomized controlled trial in the Journal of Clinical and Aesthetic Dermatology showing significant improvement in melanin index after 4 weeks of oral supplementation. The poor oral bioavailability of standard GSH has driven innovation in liposomal, sublingual, and injectable delivery methods.